Contact

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E-mail: grazyna.leszczynska@.p.lodz.pl

ORCID: https://orcid.org/0000-0002-6874-022X

 

Publications

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Synthesis of Nucleobase-Modified RNA Oligonucleotides by Post-Synthetic Approach.

 

Bartosik, K., Debiec, K., Czarnecka, A., Sochacka, E., Leszczynska, G. Molecules 2020, 25(15), 3344

 

 

C5-Substituted 2-Selenouridines Ensure Efficient Base Pairing with Guanosine; Consequences for Reading the NNG-3' Synonymous mRNA Codons.

Leszczynska, G., Cypryk, M., Gostynski, B., Sadowska, K., Herman. P., Bujacz, G., Lodyga-Chuscinska, E., Sochacka, E., Nawrot, B. Int. J. Mol. Sci. 2020, 21(8), 2882.

 

Chemical Synthesis of Oligoribonucleotide (ASL of tRNA ^Lys T. brucei) Containing a Recently Discovered Cyclic Form of 2-Methylthio-N 6 -threonylcarbamoyladenosine (ms²ct⁶A).

 

Debiec, K., Matuszewski, M., Podskoczyj, K., Leszczynska, G., Sochacka, E.  E. Chem. Eur. J. . 2019, 25(58), 13309-13317.

 

 

Reaction of S-geranyl-2-thiouracil modified oligonucleotides with alkyl amines leads to the N2-alkyl isocytosine derivatives.

Leszczynska, G., Sadowska, K., Sierant, M., Sobczak, M., Nawrot, B., Sochacka, E. Org. Biomol. Chem. 2017, 15(25), 5332-5336.

 

C5-substituents of uridines and 2-thiouridines present at the wobble position of tRNA determine the formation of their keto-enol or zwitterionic forms - a factor important for accuracy of reading of guanosine at the 3 '-end of the mRNA codons.

Sochacka, E., Lodyga-Chruscinska, E., Pawlak, J., Cypryk, M., Bartos, P., Ebenryter-Olbinska, K., Leszczynska, G., Nawrot, B. Nucleic Acids Res. 2017, 45(8), 4825-4836.

 

Post-synthetic conversion of 5-pivaloyloxymethyluridine present in a support-bound RNA oligomer into biologically relevant derivatives of 5-methyluridine.

Bartosik, K., Sochacka, E., Leszczynska, G. Org. Biomol. Chem. 2017, 15(9), 2097-2103.

 

S-Geranyl-2-thiouridine wobble nucleosides of bacterial tRNAs; chemical and enzymatic synthesis of S-geranylated-RNAs and their physicochemical characterization.

Sierant, M., Leszczynska, G., Sadowska, K., Dziergowska, A., Rozanski, M., Sochacka, E., Nawrot, B., Nucleic Acids Res. 2016, 44(22), 10986-10998.

 

Nucleoside modifications in the regulation of gene expression: focus on tRNA.

Duechler, M., Leszczynska, G., Sochacka, E., Nawrot, B, Cell Mol Life Sci. 2016, 73(16), 3075-3095.

 

S-Geranylated 2-Thiouridines of Bacterial tRNAs: Chemical Synthesis and Physicochemical Properties.

Leszczynska, G., Sadowska, K., Bartos, P., Nawrot, B., Sochacka, E. Eur. J. Org. Chem. 2016, 21, 3482-3485.

 

An efficient approach for conversion of 5-substituted 2-thiouridines built in RNA oligomers into corresponding desulfured 4-pyrimidinone products.

Chwialkowska, A., Wielgus, E., Leszczynska, G., Sobczak, M., Mikolajczyk, B., Sochacka, E., Nawrot, B. Bioorg. Med. Chem. Lett. 2015, 25(16), 3100-3104.

 

Synthesis of various substituted 5-methyluridines (xm5U) and 2-thiouridines (xm5s2U) via nucleophilic substitution of 5-pivaloyloxymethyluridine/2-thiouridine.

Bartosik, K., Leszczynska, G.,Tetrahedron Lett., 2015, 56, 6593-6597.

 

Chemical synthesis of the 5-taurinomethyl(-2-thio)uridine modified anticodon arm of the human mitochondrial tRNA^Leu(UUR) and tRNA^Lys.

Leszczyńska, G., Leonczak P., Wozniak, K., Malkiewicz, A. RNA 2014,20, 938-947.

 

Site-selected incorporation of 5-carboxymethylaminomethyl(-2-thio)uridine into RNA sequences by phosphoramidite chemistry.

Leszczynska, G., Pięta, J., Wozniak, K., Malkiewicz, A. Org. Biomol. Chem. 2014, 12, 1052-1056. 

 

Chemical synthesis of human mitochondrial tRNA^lys fragment containing 5-taurinomethyl-2-thiouridine and 6-threonylcarbamoyladenosine.

Wozniak, K., Stasiak, A., Leonczak, P., Leszczynska, G., Malkiewicz, A., Coll. Czech. Chem. Commun., Symp. Series , 2014,14, 408-410.

 

mt-tRNA components: synthesis of (2-thio)uridines modified with blocked glycine/taurine moieties at C-5,1.

Leszczynska, G., Leonczak, P., Dziergowska, A., Malkiewicz, A. Nucleosides, Nucleotides and Nucleic Acids 2013, 32, 599-616. 

 

Site-specific incorporation of 5-methylaminomethyl-2-thiouridine and 2-thiouridine(s) into RNA sequences.

Leszczynska, G., Pieta, J., Leonczak, P., Tomaszewska, A., Malkiewicz, A. Tetrahedron Lett. 2012, 53, 1214-1217.

 

Modifications modulate anticodon loop dynamics and codon recognition of E. coli tRNA^Arg1,2.

Cantara, W. A., Bilbille, Y., Kim  J., Kaiser, R., Leszczynska, G., Malkiewicz, A., Agris, P. F. J. Mol. Biol.2012, 416, 579-597.

 

Human tRNA^Lys3_UUU is pre-Structured by natural modifications for cognate and wobble codon binding through keto-enol tautomerism.

Vendeix, F. A. P, Murphy, IV F. V., Cantara, W. A., Leszczynska, G., Gustilo, E. M., Sproat, B., Malkiewicz, A., Agris, P. F. J. Mol. Biol. 2012, 416, 467-485.

 

ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA.

van den Born, E., Vågbø, C. B., Songe-Møller L., Leihne, V., Lien, G. F., Leszczynska, G., Malkiewicz, A., Krokan, H.E., Kirpekar, F., Klungland, A., Falnes, P.Ø. Nat. Commun. 2011, 172(2), 1-7.

 

Chemical synthesis of an RNA sequence containing 2-thiocytidine (s²C): the DY647 labelled anticodon stem and loop sequence of Staphylococcus aureus tRNA^Arg (s²C₃₂, mnm⁵U₃₄, t⁶A₃₇).

Leszczynska, G., Pieta, J., Sproat, B., Malkiewicz, A. Tetrahedron Lett. 2011, 52,4443-4447.

 

Functional Recognition of the Modified Human tRNA (Lys3) (UUU) Anticodon Domain by HIV's Nucleocapsid Protein and a Peptide Mimic.

Graham, W.D., Barley-Maloney, L., Stark, C.J., Kaur, A., Stolyarchuk, K., Sproat, B., Leszczynska, G., Malkiewicz, A., Safwat, N., Mucha, P., Guenther, R., Agris, P. F.  J. Mol. Biol. 2011, 410 (4), 698-715. 

 

Specificity of phage display selected peptides for modified anticodon stem and loop domains of tRNA.

 

Eshete, M., Marchbank, M.T., Deutscher, S.L., Sproat, B., Leszczynska, G., Malkiewicz, A., Agris, P.F. Protein J. 2007, 26(1), 61-73. 

 

Synthesis of modified oligonucleotides for investigation of catalytic activity and substrate specificity of DNAzyme 10-23.

Sochacka, E., Leszczyńska, G., Miskiewicz, A., Frątczak, I., Smuga, D. Ann. Pol. Chem. Soc. 2004, 652-655.

 

Modyfikowane, katalityczne kwasy nukleinowe.

Leszczyńska, G., Sochacka, E.,  Biotechnologia, 2003, 2(61), 165-181.

 

Synthesis and properties of uniquely modified oligoribonucleotides: yeast tRNA^Phe fragments with 6-methyluridine and 5,6-dimethyluridine at site-specific positions.

Sochacka, E., Czerwinska, G., Guenther, R., Cain, R., Agris, P., Małkiewicz, A. Nucleosides & Nucleotides 2000, 19(3), 515-522.

 

Structural and functional roles of the N1- and N3-protons of Yat tRNA’s position 39.

Yarian, C. S., Basti, M. M., Cain, R. J., Ansari, G., Guenther, R., Sochacka, E., Czerwińska, G., Małkiewicz, A., Agris, P. F. Nucleic Acids Res.1999, 27 (17), 3543-3549.

 

Thermodynamic conribution of nucleoside modifications to yeast tRNA^Phe anticodon stem loop analogs.

Agris, P. F., Guenther, R., Sochacka, E., Newman, W., Czerwinska, G., Liu, G., Ye, W., Malkiewicz, A. Acta Biochem. Pol. 1999, 46(1), 163 -172.